Research Technician, Cell and Molecular Biology

Target PR Range: 19-28/hr
*Depending on experience

A Research Technician position in Cell and Molecular Biology is available at the Genomic Research Center (GRC). We are seeking a highly motivated research technician to work independently and collaboratively with other scientists on projects involving human-induced pluripotent stem cell (hiPSC) reprogramming, cell differentiation, CRISPR editing, high-throughput functional screening, and a variety of molecular biology experiments in the wet lab. The successful candidate will be responsible for performing time-sensitive experiments and supporting extended hiPSC differentiation protocols, both of which require daily attention to detail. The applicant is expected to:
a. Conduct routine experimental protocols and procedures in the wet lab.
b. Maintain tissue culture, including stem cells and stem cell-derived cells.
c. Accurately record experimental setups, SOP protocols, and data quality control.
d. Maintain efficient and continuous communication with the manager and team members.
e. Support team members with routine cell culture procedures, including media preparation and reagent aliquoting.
f. Conduct molecular and cell biology experiments such as qRT-PCR, immunofluorescence, and Western blot analyses.
Qualifications
1. A bachelor’s or master’s degree in biochemistry or molecular cell biology, with at least three years of independent hands-on laboratory experience.
2. Trained and experienced in using aseptic techniques in a laminar flow biosafety cabinet.
3. Significant cell culture expertise, including maintenance, freezing, thawing, and passaging.
4. Proficiency in biochemical assays such as Western blot or WES, immunofluorescence, and flow cytometry.
5. Skilled in molecular biology techniques such as RNA extraction, cDNA synthesis, and RT-qPCR.
6. Knowledge of serial dilution and calculation of solution concentration.
7. Excellent written and oral communication skills.
8. Organized in planning long experimental protocols that may sometimes require working on weekends.
9. Nice to have: Experience with culturing human iPSC and differentiating iPSC into brain cell types.